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| Registros recuperados : 4 | |
| 1. |  | VAMVAKA, E.; TWYMAN, R. M.; MURAD, A. M.; MELNIK, S.; TEH, A. Y.; ARCALIS, E.; ALTMANN, F.; STOGER, E.; RECH, E.; MA, J. K. C.; CHRISTOU, P.; CAPELL, T. Rice endosperm produces an underglycosylated and potent form of the HIV-neutralizing monoclonal antibody 2G12. Plant Biotechnology Journal, p. 1-12, 2015.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 2. | | MUÑOZ-BASAGOITIA, J.; MONTEIRO, F. L. L.; KRUMPE, L. R. H.; ARMARIO-NAJERA, V.; SHENOY, S. R.; PEREZ-ZSOLT, D.; WESTGARTH, H. J.; VILLORBINA, G.; BOMFIM, L. M.; RAÏCH-REGUÉ, D.; NOGUERAS, L.; HENRICH, C. J.; GALLEMÍ, M.; MOREIRA, F. R. R.; TORRES, P.; WILSON, J.; D’ARC, M.; MARFIL, S.; HERLINGER, A. L.; PRADENAS, E.; HIGA, L. M.; PORTERO-OTIN, M.; TRINITÉ, B.; TWYMAN, R. M; CAPELL, T.; TANURI, A.; BLANCO, J.; IZQUIERDO-USEROS, N.; RECH FILHO, E. L.; CHRISTOU, P.; O’KEEFE, B. R. Cyanovirin-N binds to select SARS-CoV-2 spike oligosaccharides outside of the receptor binding domain and blocks infection by SARS-CoV-2. Proceedings of the National Academy of Sciences (PNAS), v. 120, n. 10, 2023. e2214561120. Na publicação: Elibio L. Rech.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 3. | | GÓMEZ, M. L.; HUANG, X.; ALVAREZ, D.; HE, W.; BAYSAL, C.; ZHU, C.; ARMARIO-NAJERA, V.; PERERA, A. B.; BENNASSER, P. C.; SABA-MAYORAL, A.; SOBRINO-MENGUAL, G.; VARGHEESE, A.; ABRANCHES, R.; ABREU, I. A.; BALAMURUGAN, S.; BOCK, R.; BUYEL, J. F.; CUNHA, N. B. da; DANIELL, H.; FALLER, R.; FOLGADO, A.; GOWTHAM, I.; HÄKKINEN, S. T.; KUMAR, S.; RAMALINGAM, S. K.; LACORTE, C. C.; LOMONOSSOFF, G. P.; LUÍS, I. M.; MA, J. K.-C.; MCDONALD, K. A.; MURAD, A. M.; NANDI, S.; O'KEEFE, B.; OKSMAN-CALDENTEY, K.-M.; PARTHIBAN, S.; PAUL, M. J.; PONNDORF, D.; RECH FILHO, E. L.; RODRIGUES, J. C. M.; RUF, S.; SCHILLBERG, S.; SCHWESTKA, J.; SHAH, P. S.; SINGH, R.; STOGER, E.; TWYMAN, R. M.; VARGHESE, I. P.; VIANNA, G. R.; WEBSTER, G.; WILBERS, R. H. P.; CAPELL, T.; CHRISTOU, P. Contributions of the international plant science community to the fight against human infectious diseases - part 1: epidemic and pandemic diseases. Plant Biotechnology Journal, v. 19, p. 1901-1920, 2021. Na publicação: Cristiano Lacorte; Andre Murad; Elibio Rech.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 4. | | HE, W.; BAYSAL, C.; GÓMEZ, M. L.; HUANG, X.; ALVAREZ, D.; ZHU, C.; ARMARIO-NAJERA, V.; PERERA, A. B.; BENNASER, P. C.; SABA-MAYORAL, A.; SOBRINO-MENGUAL, G.; VARGHEESE, A.; ABRANCHES, R.; ABREU, I. A.; BALAMURUGAN, S.; BOCK, R.; BUYEL, J. F.; CUNHA, N. B. da; DANIELL, H.; FALLER, R.; FOLGADO, A.; GOWTHAM, I.; HÄKKINEN, S. T.; KUMAR, S.; KUMAR, R. S.; LACORTE, C. C.; LOMONOSSOFF, G. P.; LUÍS, I. M.; MA, J. K.-C.; MCDONALD, K. A.; MURAD, A. M.; NANDI, S.; O’KEEF, B.; PARTHIBAN, S.; PAUL, M. J.; PONNDORF, D.; RECH FILHO, E. L.; RODRIGUES, J. C. M.; RUF, S.; SCHILLBERG, S.; SCHWESTKA, J.; SHAH, P. S.; SINGH, R.; STOGER, E.; TWYMAN, R. M.; VARGHESE, I. P.; VIANNA, G. R.; WEBSTER, G.; WILBERS, R. H. P.; CHRISTOU, P.; OKSMAN-CALDENTEY, K.-M.; CAPELL, T. Contributions of the international plant science community to the fight against infectious diseases in humans - part 2: Affordable drugs in edible plants for endemic and re-emerging diseases. Plant Biotechnology Journal, v. 19, p. 1921-1936, 2021. Na publicação: Cristiano Lacorte; Andre Murad; Elibio Rech.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 4 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
08/09/2022 |
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Data da última atualização: |
08/09/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
FERREIRA, E. M.; ROMERO, L. C.; CUNHA, M. DE L. R. DE S. DA; MALAGO JUNIOR, W.; CAMARGO, C. H.; BARIONI JUNIOR, W.; ZAFALON, L. F. |
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Afiliação: |
ELKA MACHADO FERREIRA, UNESP; LETÍCIA CASTILHO ROMERO, UNESP; MARIA DE LOURDES RIBEIRO DE SOUZA DA CUNHA, UNESP; WILSON MALAGO JUNIOR, CPPSE; CARLOS HENRIQUE CAMARGO, Bacteriology Center, Adolfo Lutz Institute; WALDOMIRO BARIONI JUNIOR, CPPSE; LUIZ FRANCISCO ZAFALON, CPPSE. |
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Título: |
Persistence of Staphylococcus spp. in milk from cows undergoing homeopathy to control subclinical mastitis. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
BMC Veterinary Research, v. 18, n. 273, 2022. |
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Páginas: |
13 p. |
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DOI: |
https://doi.org/10.1186/s12917-022-03364-8 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Mastitis is one of the major diseases in dairy cattle, as it causes great economic losses to producers due to the reduction of milk production and changes in the quality of the product. The disease is mainly caused by bacteria of the genus Staphylococcus spp., these microorganisms can express various virulence factors, such as biofilms for example. In herds with organic management, producers and technicians use unconventional ways to treat and control the disease, such as homeopathy. However, it is not known if this type of treatment is able to control pathogenic bacteria such as those of the genus Staphylococcus, of relevance to animal and human health. Thus, the objective of this study was to investigate the production of biofilm in vitro and its genes by Staphylococcus spp. isolated in the milk of cows treated with homeopathy, as well as the persistence of microorganisms in animals. Methods: Ninety-nine isolates of Staphylococcus spp. from cows treated and not treated with homeopathy were identified by internal transcribed space-polymerase chain reaction and investigated for the presence of the icaABCD, bap, aap, atlE, and bhp genes and in vitro biofilm production using the adhesion method on polystyrene plates. The enzyme restriction profile was determined by Pulsed-Field Gel Electrophoresis. Clusters of S. aureus and S. epidermidis with three or more isolates had an isolate selected for Multilocus Sequence Typing. Results: The frequency of S. aureus isolations was similar in treated and untreated cows, while 71.4% of the coagulase-negative identified were isolated in cows treated with homeopathy. The distribution of the operon ica genes was similar in animals with and without treatment, except for the icaD gene, more frequent in treated cows. Production of biofilm was associated with presence of one or more genes from the icaADBC operon. S. aureus revealed a greater diversity and greater dissemination in cows treated and not treated with homeopathy. Sequence Types ST1, ST5, and ST126 were identified in S. aureus. Conclusions: The presence of biofilm-associated genes and the in vitro production of biofilms, combined with the persistence of clonal profiles of Staphylococcus spp. demonstrate other forms of control for bovine mastitis should be researched for organic production herds. MenosBackground: Mastitis is one of the major diseases in dairy cattle, as it causes great economic losses to producers due to the reduction of milk production and changes in the quality of the product. The disease is mainly caused by bacteria of the genus Staphylococcus spp., these microorganisms can express various virulence factors, such as biofilms for example. In herds with organic management, producers and technicians use unconventional ways to treat and control the disease, such as homeopathy. However, it is not known if this type of treatment is able to control pathogenic bacteria such as those of the genus Staphylococcus, of relevance to animal and human health. Thus, the objective of this study was to investigate the production of biofilm in vitro and its genes by Staphylococcus spp. isolated in the milk of cows treated with homeopathy, as well as the persistence of microorganisms in animals. Methods: Ninety-nine isolates of Staphylococcus spp. from cows treated and not treated with homeopathy were identified by internal transcribed space-polymerase chain reaction and investigated for the presence of the icaABCD, bap, aap, atlE, and bhp genes and in vitro biofilm production using the adhesion method on polystyrene plates. The enzyme restriction profile was determined by Pulsed-Field Gel Electrophoresis. Clusters of S. aureus and S. epidermidis with three or more isolates had an isolate selected for Multilocus Sequence Typing. Results: The frequency of S. aureus isolatio... Mostrar Tudo |
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Palavras-Chave: |
Clonal profile; IcaADBC. |
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Thesaurus NAL: |
Biofilm. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1146229/1/PersistenceStaphylococcusMilk.pdf
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Marc: |
LEADER 03109naa a2200253 a 4500
001 2146229
005 2022-09-08
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1186/s12917-022-03364-8$2DOI
100 1 $aFERREIRA, E. M.
245 $aPersistence of Staphylococcus spp. in milk from cows undergoing homeopathy to control subclinical mastitis.$h[electronic resource]
260 $c2022
300 $a13 p.
520 $aBackground: Mastitis is one of the major diseases in dairy cattle, as it causes great economic losses to producers due to the reduction of milk production and changes in the quality of the product. The disease is mainly caused by bacteria of the genus Staphylococcus spp., these microorganisms can express various virulence factors, such as biofilms for example. In herds with organic management, producers and technicians use unconventional ways to treat and control the disease, such as homeopathy. However, it is not known if this type of treatment is able to control pathogenic bacteria such as those of the genus Staphylococcus, of relevance to animal and human health. Thus, the objective of this study was to investigate the production of biofilm in vitro and its genes by Staphylococcus spp. isolated in the milk of cows treated with homeopathy, as well as the persistence of microorganisms in animals. Methods: Ninety-nine isolates of Staphylococcus spp. from cows treated and not treated with homeopathy were identified by internal transcribed space-polymerase chain reaction and investigated for the presence of the icaABCD, bap, aap, atlE, and bhp genes and in vitro biofilm production using the adhesion method on polystyrene plates. The enzyme restriction profile was determined by Pulsed-Field Gel Electrophoresis. Clusters of S. aureus and S. epidermidis with three or more isolates had an isolate selected for Multilocus Sequence Typing. Results: The frequency of S. aureus isolations was similar in treated and untreated cows, while 71.4% of the coagulase-negative identified were isolated in cows treated with homeopathy. The distribution of the operon ica genes was similar in animals with and without treatment, except for the icaD gene, more frequent in treated cows. Production of biofilm was associated with presence of one or more genes from the icaADBC operon. S. aureus revealed a greater diversity and greater dissemination in cows treated and not treated with homeopathy. Sequence Types ST1, ST5, and ST126 were identified in S. aureus. Conclusions: The presence of biofilm-associated genes and the in vitro production of biofilms, combined with the persistence of clonal profiles of Staphylococcus spp. demonstrate other forms of control for bovine mastitis should be researched for organic production herds.
650 $aBiofilm
653 $aClonal profile
653 $aIcaADBC
700 1 $aROMERO, L. C.
700 1 $aCUNHA, M. DE L. R. DE S. DA
700 1 $aMALAGO JUNIOR, W.
700 1 $aCAMARGO, C. H.
700 1 $aBARIONI JUNIOR, W.
700 1 $aZAFALON, L. F.
773 $tBMC Veterinary Research$gv. 18, n. 273, 2022.
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Embrapa Pecuária Sudeste (CPPSE) |
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